Gotaq g2 green master mix contains two dyes blue and yellow that separate during electrophoresis to indicate migration progress. Gotaq g2 hot start green master mix protocolpdf promega. It is best for routine pcr applications from templates including pure dna solutions, bacterial colonies, and cdna. Secondgeneration, highperformance gotaq g2 dna polymerase with mgfree buffers. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Overview protocols specifications resources set up pcr in less than a minute with convenient master mixes gotaq dna polymerase is a proprietary. Gotaq colorless master mix has the same formulation as the gotaq green master mix but does not include the dyes. Links to the entire contents of the promega product catalog are accessible from this page. Gotaq hot start polymerase 100u prm5001 500u prm5005 2,500u prm5006 10,000u prm5008 gotaq green master mix 100 reactions prm7122 1,000 reactions prm7123 gotaq colorless master mix 100 reactions prm72 1,000 reactions prm73 gotaq flexi dna polymerase 100u prm8291 500u prm8295 2,500u prm8296 prm8297 10,000u prm8298. Use gotaq hot start green and colorless master mixes for easy.
For the gotaq green master mix amplifications, the provided buffer contained mgcl 2 1. This premixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for pcr set up. Both mixes contain gotaq hot start polymerase, which can be used for hotstart pcr. Stay notified of promega events, products and news. Gotaq green master mix is a premixed readytouse solution containing bacterially derived taq dna.
In conclusion, promegas gotaq green master mix is very useful and convenient especially when running a large number of pcrs in a short time. Gotaq green master mix contains two dyes blue and yellow that allow monitoring of progress during electrophoresis. Gotaq dna polymerasea,b is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Gotaq green master mix for quick and easy twostep rtpcr. Green gotaq reaction buffer is a proprietary buffer containing a compound that increases sample density, and yellow and blue dyes, which function as loading. Reactions assembled with the gotaq master mixes have sufficient density for direct loading onto agarose gels. Pcr materials highfidelity pcr master mix phusion forward. Gotaq green and colorless master mix reaction size is based on a 50l reaction volume.
Gotaq green master mix allows the researcher to move directly from the reverse transcription reaction to pcr then directly to agarose gel analysis, enabling rapid screening of many samples. Goscript reverse transcription system ohio university. A master mix is a mixture containing precursors and enzymes used as an ingredient in rtpcr techniques in molecular biology. Gotaq green master mixa is a premixed readytouse solution containing bacterially derived taq dna. The green master mix allows direct gel analysis after amplification. Thermo scientific 2 dreamtaq hot start green pcr master. Amplification products 160 bp, 727 bp, 2 kb, or 5 kb from human genomic dna are shown in the figure above. Gotaq hot start green master mix and gotaq hot start colorless master mix are two new pcr master mixes offered by promega. Pdf 204 kb gotaq colorless master mix m7 product information. I am trying to identify flanking sequence for insertion site, using adapter method, so since i check the protocol, the master mix of the pcr reaction includes. Get a one time, introductory discount of 35% on gotaq qpcr master mix.
It is essential to use proper techniques when isolating and. Dreamtaq hot start dna polymerase amplifies from lower template amounts than hotstart dna polymerases from other suppliers. Gotaq dna polymerase is a proprietary formulation of taq dna polymerase that gives robust amplification equal to and, in some cases, superior to that of standard taq dna polymerase for ultimate in convenience, choose gotaq colorless master mix or gotaq green master mix and directly substitute in your current pcr application. Thermo scientific pcr master mix is a 2x concentrated solution of taq dna polymerase, dntps, and all of the components required for pcr, except dna template and primers. Dreamtaq green pcr master mix 2x thermo fisher scientific. Sybr green master mixes powertrack sybr green master mix powerup sybrmaster mix luminariscolor higreen qpcr master mix, low rox biorad ssoadvanced universal sybr green supermix takara tb green premix ex taq ii tli rnase h plus promega gotaq qpcr master mix kapa sybr fast qpcr kits. M712 gotaq green master mix p119 nucleasefree water. Taq 5x master mix is an optimized readytouse solution containing taq dna polymerase, dntps, mgcl 2, kcl and stabilizers.
The master mixes contain buffer, dntps, thermostable hotstart dna polymerase, and sybr green dyeeverything needed for realtime pcr. Dreamtaq hot start dna polymerase thermo fisher scientific. Readytouse master mix format with gotaq dna polymerase. Gotaq green master mix m712 protocol pdf 205 kb english gotaq colorless master mix m7 product information pdf 205 kb english specifications. The 5x green gotaq reaction buffer contains two dyes blue and yellow. Standard dna polymerase assay conditions not pcr conditions. Gotaq g2 master mixes are premixed readytouse solutions containing gotaq g2 dna polymerase, dntps, mgcl2 and reaction buffers at optimal concentrations for efficient amplification of a wide range of dna templates by pcr. Gotaq green and colorless master mixes exhibit 53 exonuclease activity. Can i use go taq green master mix for pcr, in adapter method.
New formulation of taq dna polymerase with enhanced performance over standard taq dna polymerase. In all pipetting steps, be sure to change the pipette tip each time an operation is completed. Promega go taq master mixes gotaq green master mix. Gotaq g2 green master mix product informationpdf 207 kb english. Both master mixes are readytouse 2x solutions that contain gotaq dna polymerase, dntps, mgcl 2 and reaction buffer at optimal concentrations for. Gotaq green master mix m712 protocol pdf 205 kb english. Gotaq green master mix the wolfson centre for applied structural. Readytouse optimized master mix for roomtemperature pcr assembly. Gotaq green master mix m712 protocolpdf 205 kb english gotaq colorless. Highperformance hotstart gotaq g2 enzyme with convenient roomtemperature setup. Prepare one of the following reaction mixes on ice. Dna and rna extraction, plasmid purification, reporter assays, pcr, qpcr, cloning enzymes, cell viability assays, apoptosis detection systems, luminometers, protein expression systems and more. M of forward common fc and reverse common rc primer of each gene, and approximately 50 ng of genomic dna for the amplification of each gene.
Gotaq g2 green master mix contains two dyes blue and yellow that allow monitoring of progress during electrophoresis. You must order the special promotion catalog number above to receive the promotion price. We compared the ability of the two enzymes to amplify seven dna targets. Set up pcr in less than a minute with convenient master mixes. Get a one time, introductory discount of 35% on gotaq qpcr. Gotaq green master mix is a premixed readytouse solution containing bacterially derived taq dna polymerase, dntps, mgcl 2 and reaction buffers at optimal concentrations for efficient amplification of dna templates by pcr. Such mixtures contain a mixture dntps required as a substrate for the building of new dna strands, mgcl 2, taq polymerase an enzyme required to building new dna strands, a ph buffer and come mixed in nucleasefree water master mixes for realtime pcr include. Gotaq green master mix m7122 pdf 66 kb english united.
Sybr green realtime pcr master mixes thermo fisher. The bright choice for dyebased qpcr abstract the new gotaq qpcr master mix is a 2x master mix for quantitative pcr qpcr and features gotaq hot start polymerase, a fulllength taqdna polymerase bound to a proprietary antibody that prevents polymerase activity at room temperature. Gotaq colorless master mix m7 product information pdf 205 kb english. It includes dreamtaq hot start dna polymerase, 2x dreamtaq green buffer, datp, dctp, dgtp and dttp, 0. Thaw the gotaq green master mix at room temperature. The master mix format gives you the advantage of easy reaction assembly. Pcr reagents are kits that include researchersupplied components, such as purified nucleic acid templates and oligonucleotide primers, along with components available through vwr, such as deoxyribonucleotides dntps, and dna polymerase. Gotaq green master mix product information promega corporation.
Such mixtures contain a mixture dntps required as a substrate for the building of new dna strands, mgcl 2, taq polymerase an enzyme required to building new dna strands, a ph buffer and come mixed in nucleasefree water. Can anyone recommend a protocol for sybr green master mix. Dreamtaq hot start dna polymerase produces more product, cleaner bands, and longer amplicons than hotstart dna polymerases from other suppliers. Gotaq g2 hot start taq is available as a master mix or as a standalone enzyme, it is supplied with 5x green gotaq flexi buffer, 5x colorless gotaq flexi buffer and 25mm mgcl 2. Pdf 203 kb certificate of analysis lookup certificate of analysis storage conditions30c to 10c. Powertrack sybr green master mix, 5pack 5 x 5 ml 2,500 reactions a46111 powertrack sybr green master mix, 10pack 10 x 5 ml 5,000 reactions a46112 powertrack sybr green master mix, bulk pack 1 x 50 ml 5,000 reactions a461 broad instrument compatibility powertrack sybr green master mix can be used in either. The new gotaq qpcr master mix is a 2x master mix for quantitative pcr qpcr and features gotaq hot start polymerase, a fulllength taq dna polymerase bound to a proprietary antibody that prevents polymerase activity at room temperature. Fyi, the amplification efficiency for all primers have been validated using the commercial sybr green master mix, and were running our samples on a cfx96 realtime detection system biorad. Gotaq g2 green master mix contains two dyes blue and yellow that allow.
Applied biosystems powertrack sybr green master mix. Thermo scientific 2 dreamtaq hot start green pcr master mix 2x. Nucleic acid purification and quality assessment are important steps in the pcr workflow. Gotaq g2 master mixes pcr master mix promega corporation. Gotaq g2 green and colorless master mixes are readytouse, green and colorless, 2x master mixes containing all necessary components for robust, reliable pcr. Applied biosystems sybr green master mixes are designed for quantitative realtime pcr using a set of two pcr primers that flank the target region. Gotaq green master mix protocol promega corporation. It ensures higher sensitivity, longer pcr products and higher yields compared to conventional taq dna polymerase. The gotaq green master mix performance was similar to or better than standard taq dna polymerase in the amplification reactions described here. Each set of pcr reactions contained either 3 pg, 30 pg, or 3 ng. The 5x green gotaq reaction buffer contains two dyes blue and yellow that. I strongly recommend reading the protocol before setting up pcr reactions using gotaq green master mix. Gotaq qpcr master mix is a fast, sensitive and reliable 2x master mix optimized for quantitative realtime pcr.
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